PrimePCR™ SYBR® Green Assay: ASAH2B, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0041043

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: ASAH2B, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ASAH2B, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0038321
Assay Design:   Intron-spanning
Chromosome Location:   10:52512228-52514360question
Amplicon Length:   109
Splice Variants Targeted:   ENST00000445041 ENST00000555262 ENST00000360167 ENST00000449574 ENST00000395526 ENST00000329428 ENST00000447815 ENST00000443575 ENST00000374007 ENST00000374006 ENST00000185907 ENST00000404991 ENST00000420079 ENST00000426610 ENST00000374647 ENST00000341500

Gene Information

Description Not Available

Gene Symbol:   ASAH2B
Gene Name:   N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2B
Aliases:   ASAH2C, ASAH2L, bA449O16.3
RefSeq:   NC_000010.10 NT_030059.13
Ensembl:   ENSG00000204147
Entrez:   653308
Chromosome Mapping:   10q11.23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998400
y-intercept 35.470000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download