PrimePCR™ SYBR® Green Assay: AL662899.1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCIP0041183


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PrimePCR™ PreAmp for SYBR® Green Assay: AL662899.1, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: AL662899.1, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCID0038461
Assay Design:		Intron-spanning
Chromosome Location:		6:31680461-31681581question
Amplicon Length:		120
Splice Variants Targeted:		ENST00000543610 ENST00000449103 ENST00000359337 ENST00000359882 ENST00000355961 ENST00000361988 ENST00000326194 ENST00000537758 ENST00000355092 ENST00000367574 ENST00000469626 ENST00000435051 ENST00000431420 ENST00000548848 ENST00000435369 ENST00000434670 ENST00000547676 ENST00000348639 ENST00000448277 ENST00000539789 ENST00000426726 ENST00000409239 ENST00000450147 ENST00000399142 ENST00000383418 ENST00000419767 ENST00000431644 ENST00000546405 ENST00000419000 ENST00000453077 ENST00000550508 ENST

Gene Information

Description Not Available

Gene Symbol:		AL662899.1
Gene Name:		Lymphocyte antigen 6 complex, locus G6ELymphocyte antigen 6 complex, locus G6E, isoform CRA_aUncharacterized protein
Aliases:		Not Available
RefSeq:		Not Available
Ensembl:		ENSG00000255552
Chromosome Mapping:		Not Available

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.998900
y-intercept	36.070000
Efficiency	100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.