PrimePCR™ SYBR® Green Assay: SIVA1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0041335

List Price:    $153.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0038613
Assay Design:   Intron-spanning
Chromosome Location:   14:105222955-105225813question
Amplicon Length:   162
Splice Variants Targeted:   ENST00000556195 ENST00000347067 ENST00000329967

Gene Information

This gene encodes a protein with an important role in the apoptotic (programmed cell death) pathway induced by the CD27 antigen a member of the tumor necrosis factor receptor (TFNR) superfamily. The CD27 antigen cytoplasmic tail binds to the N-terminus of this protein. Two alternatively spliced transcript variants encoding distinct proteins have been described. [provided by RefSeq Jul 2008]

Gene Symbol:   SIVA1
Gene Name:   SIVA1, apoptosis-inducing factor
Aliases:   CD27BP, SIVA, Siva-1, Siva-2
RefSeq:   NC_000014.8 NT_026437.12
Ensembl:   ENSG00000184990
Entrez:   10572
Chromosome Mapping:   14q32.33

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 36.030000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download