PrimePCR™ SYBR® Green Assay: SEPSECS, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCIP0041604


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PrimePCR™ PreAmp for SYBR® Green Assay: SEPSECS, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: SEPSECS, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCID0038882
Assay Design:		Intron-spanning
Chromosome Location:		4:25153618-25156696question
Amplicon Length:		114
Splice Variants Targeted:		ENST00000382103 ENST00000302922

Gene Information

The amino acid selenocysteine is the only amino acid that does not have its own tRNA synthetase. Instead this amino acid is synthesized on its cognate tRNA in a three step process. The protein encoded by this gene catalyzes the third step in the process the conversion of O-phosphoseryl-tRNA(Sec) to selenocysteinyl-tRNA(Sec).[provided by RefSeq Mar 2011]

Gene Symbol:		SEPSECS
Gene Name:		Sep (O-phosphoserine) tRNA:Sec (selenocysteine) tRNA synthase
Aliases:		DKFZp434B1417, LP, MGC161491, SLA, SLA/LP
RefSeq:		NC_000004.11 NT_006316.16
Ensembl:		ENSG00000109618
Entrez:		51091
Chromosome Mapping:		4p15.2

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.997900
y-intercept	36.260000
Efficiency	101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.