PrimePCR™ SYBR® Green Assay: TLE2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCIP0041720


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PrimePCR™ PreAmp for SYBR® Green Assay: TLE2, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: TLE2, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCID0038998
Assay Design:		Intron-spanning
Chromosome Location:		19:3027827-3028782question
Amplicon Length:		158
Splice Variants Targeted:		ENST00000262953 ENST00000591529 ENST00000443826 ENST00000590536 ENST00000591457 ENST00000587137 ENST00000592584 ENST00000455444 ENST00000426948

Gene Information

Description Not Available

Gene Symbol:		TLE2
Gene Name:		transducin-like enhancer of split 2 (E(sp1) homolog, Drosophila)
Aliases:		ESG, ESG2, FLJ41188, GRG2
RefSeq:		NC_000019.9 NT_011255.14
Ensembl:		ENSG00000065717
Entrez:		7089
Chromosome Mapping:		19p13.3

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999500
y-intercept	36.170000
Efficiency	97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.