PrimePCR™ lncRNA SYBR® Green Assay:LINC00298, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression.

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qhsaLEP0140029

List Price:    $174.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   lncRNA Expression
Unique Assay ID:   qhsaLED0062789
Assay Design:   exonic
Chromosome Location:   chr2:7968273-7976818question
Amplicon Length:   87
Refseq Transcripts Amplified:   NR_015405
Splice Variants Targeted:   ENST00000456681 ENST00000426969
LNCipedia Splice Variants Targeted:   LINC00298:6 LINC00298:1 LINC00298:3 LINC00298:4 LINC00298:5

Gene Information

Not Available

Gene Symbol:   LINC00298
Gene Name:   long intergenic non-protein coding RNA 298 [Source:HGNC Symbol;Acc:HGNC:49257]
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSG00000235665
LNCipedia:   LINC00298
Entrez:   339788
Chromosome Mapping:   2p25.1
Gene Strand:   Reverse strand

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 36.480000
Efficiency 1.95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download