PrimePCR™ lncRNA SYBR® Green Assay:EGOT, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression.

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info: Same primer pair as used in probe assay qhsaLEP0143704. May amplify unspliced transcripts of an antisense gene.


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PrimePCR™ IncRNA PreAmp for SYBR® Green Assay: EGOT, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ IncRNA Template for SYBR® Green Assay: EGOT, Human
Reaction: 4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		lncRNA Expression
Unique Assay ID:		qhsaLED0066464
Assay Design:		exonic
Chromosome Location:		chr3:4749580-4749693question
Amplicon Length:		84
Refseq Transcripts Amplified:		NR_004428
Splice Variants Targeted:		ENST00000414938
LNCipedia Splice Variants Targeted:		EGOT:3 EGOT:2

Gene Information

Not Available

Gene Symbol:		EGOT
Gene Name:		eosinophil granule ontogeny transcript (non-protein coding) [Source:HGNC Symbol;Acc:HGNC:37129]
Aliases:		EGO, NCRNA00190
RefSeq:		Not Available
Ensembl:		ENSG00000235947
LNCipedia:		EGOT
Entrez:		100126791
Chromosome Mapping:		3p26.1
Gene Strand:		Reverse strand

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999800
y-intercept	35.950001
Efficiency	1.99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.