PrimePCR™ lncRNA SYBR® Green Assay:RP11-525K10.3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression.

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qhsaLEP0147382

List Price:    $174.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   lncRNA Expression
Unique Assay ID:   qhsaLED0070142
Assay Design:   exonic
Chromosome Location:   chr16:80528656-80540850question
Amplicon Length:   102
Refseq Transcripts Amplified:   NR_120307
Splice Variants Targeted:   ENST00000563267 ENST00000568552 ENST00000568776 ENST00000564411 ENST00000568819 ENST00000565050
LNCipedia Splice Variants Targeted:   lnc-CDYL2-6:3 lnc-CDYL2-6:2 lnc-CDYL2-6:6 lnc-CDYL2-6:7 lnc-CDYL2-6:4 lnc-CDYL2-6:1 lnc-CDYL2-6:8 lnc-CDYL2-6:5

Gene Information

Not Available

Gene Symbol:   RP11-525K10.3
Gene Name:   Not Available
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSG00000259867
LNCipedia:   lnc-CDYL2-6
Chromosome Mapping:   Not Available
Gene Strand:   Reverse strand

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.889999
Efficiency 1.95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download