PrimePCR™ lncRNA SYBR® Green Assay:FLJ40194, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression.

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qhsaLEP0148790

List Price:    $174.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   lncRNA Expression
Unique Assay ID:   qhsaLED0071550
Assay Design:   exonic
Chromosome Location:   chr17:49252573-49252672question
Amplicon Length:   70
Refseq Transcripts Amplified:   NR_034161
Splice Variants Targeted:   ENST00000322227 ENST00000502951 ENST00000511008
LNCipedia Splice Variants Targeted:   lnc-ABI3-2:8 lnc-ABI3-2:6 lnc-ABI3-2:7 lnc-ABI3-2:1 lnc-ABI3-2:2 lnc-ABI3-2:3 lnc-ABI3-2:4 lnc-ABI3-2:5 lnc-ABI3-2:9

Gene Information

Not Available

Gene Symbol:   FLJ40194
Gene Name:   uncharacterized FLJ40194 [Source:EntrezGene;Acc:124871]
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSG00000177369
LNCipedia:   lnc-ABI3-2
Entrez:   124871
Chromosome Mapping:   17q21.32
Gene Strand:   Forward strand

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998600
y-intercept 35.000000
Efficiency 2

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download