PrimePCR™ lncRNA SYBR® Green Assay:SNHG12, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression.

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info: Same primer pair as used in probe assay qhsaLEP0149122


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PrimePCR™ IncRNA PreAmp for SYBR® Green Assay: SNHG12, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ IncRNA Template for SYBR® Green Assay: SNHG12, Human
Reaction: 4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		lncRNA Expression
Unique Assay ID:		qhsaLED0071882
Assay Design:		exonic
Chromosome Location:		chr1:28579963-28580641question
Amplicon Length:		71
Refseq Transcripts Amplified:		NR_024127
Splice Variants Targeted:		ENST00000464612 ENST00000481368 ENST00000474814 ENST00000475441 ENST00000488745 ENST00000531126 ENST00000461448 ENST00000461832 ENST00000483436 ENST00000464115 ENST00000470977 ENST00000481220
LNCipedia Splice Variants Targeted:		SNHG12:15 SNHG12:14 SNHG12:1 SNHG12:10 SNHG12:6 SNHG12:4 SNHG12:5 SNHG12:11 SNHG12:12 SNHG12:2 SNHG12:9 SNHG12:8 SNHG12:7 SNHG12:13 SNHG12:3

Gene Information

Not Available

Gene Symbol:		SNHG12
Gene Name:		small nucleolar RNA host gene 12 [Source:HGNC Symbol;Acc:HGNC:30062]
Aliases:		ASLNC04080, C1orf79, LINC00100, NCRNA00100
RefSeq:		Not Available
Ensembl:		ENSG00000197989
LNCipedia:		SNHG12
Entrez:		85028
Chromosome Mapping:		1p35.3
Gene Strand:		Reverse strand

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999700
y-intercept	35.480000
Efficiency	1.97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.