PrimePCR™ lncRNA SYBR® Green Assay:HAND2-AS1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression.

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qhsaLEP0151636

List Price:    $174.00
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PrimePCR™ IncRNA PreAmp for SYBR® Green Assay: HAND2-AS1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ IncRNA Template for SYBR® Green Assay: HAND2-AS1, Human
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   lncRNA Expression
Unique Assay ID:   qhsaLED0074396
Assay Design:   exonic
Chromosome Location:   chr4:173536848-173536965question
Amplicon Length:   88
Refseq Transcripts Amplified:  
Splice Variants Targeted:   ENST00000512246 ENST00000509866 ENST00000502896 ENST00000514673 ENST00000507062 ENST00000509640 ENST00000503198 ENST00000503474 ENST00000512943 ENST00000514431 ENST00000511728 ENST00000511196 ENST00000507322 ENST00000502334 ENST00000515741 ENST00000508534 ENST00000507571 ENST00000515350 ENST00000510339 ENST00000505621 ENST00000507636 ENST00000512099 ENST00000515310 ENST00000515345 ENST00000510268 ENST00000616485 ENST00000502941 ENST00000510221 ENST00000504429 ENST00000508887 ENST00000515376
LNCipedia Splice Variants Targeted:   HAND2-AS1:22 HAND2-AS1:37 HAND2-AS1:14 HAND2-AS1:15 HAND2-AS1:38 HAND2-AS1:44 HAND2-AS1:36 HAND2-AS1:16 HAND2-AS1:23 HAND2-AS1:45 HAND2-AS1:28 HAND2-AS1:29 HAND2-AS1:3 HAND2-AS1:4 HAND2-AS1:18 HAND2-AS1:9 HAND2-AS1:21 HAND2-AS1:30 HAND2-AS1:42 HAND2-AS1:41 HAND2-AS1:40 HAND2-AS1:26 HAND2-AS1:24 HAND2-AS1:12 HAND2-AS1:13 HAND2-AS1:27 HAND2-AS1:8 HAND2-AS1:11 HAND2-AS1:20 HAND2-AS1:10 HAND2-AS1:6 HAND2-AS1:7 HAND2-AS1:17 HAND2-AS1:1 HAND2-AS1:2 HAND2-AS1:31 HAND2-AS1:43

Gene Information

Not Available

Gene Symbol:   HAND2-AS1
Gene Name:   HAND2 antisense RNA 1 (head to head) [Source:HGNC Symbol;Acc:HGNC:48872]
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSG00000237125
LNCipedia:   HAND2-AS1
Chromosome Mapping:   Not Available
Gene Strand:   Forward strand

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 36.000000
Efficiency 1.93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download