PrimePCR™ lncRNA SYBR® Green Assay:CLUHP3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression.

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qhsaLEP0179971

List Price:    $169.00
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PrimePCR™ IncRNA PreAmp for SYBR® Green Assay: CLUHP3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ IncRNA Template for SYBR® Green Assay: CLUHP3, Human
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   lncRNA Expression
Unique Assay ID:   qhsaLED0102731
Assay Design:   exonic
Chromosome Location:   chr16:31705467-31705761question
Amplicon Length:   66
Refseq Transcripts Amplified:   NR_024034
Splice Variants Targeted:   ENST00000411844 ENST00000431761 ENST00000254109 ENST00000532304 ENST00000525610 ENST00000563678
LNCipedia Splice Variants Targeted:   lnc-ZNF720-1:2 lnc-ZNF720-1:5 lnc-ZNF720-1:4 lnc-ZNF720-1:1 lnc-ZNF720-1:8 lnc-ZNF720-1:3 lnc-ZNF720-1:7 lnc-ZNF720-1:6

Gene Information

Not Available

Gene Symbol:   CLUHP3
Gene Name:   clustered mitochondria (cluA/CLU1) homolog pseudogene 3 [Source:HGNC Symbol;Acc:HGNC:28447]
Aliases:   C16orf67, KIAA0664L3, KIAA0664P3, URLC3
RefSeq:   Not Available
Ensembl:   ENSG00000131797
LNCipedia:   lnc-ZNF720-1
Entrez:   100132341
Chromosome Mapping:   16p11.2
Gene Strand:   Forward strand

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 36.250000
Efficiency 1.97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download