PrimePCR™ lncRNA SYBR® Green Assay:lnc-ACTA2-1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression.

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qhsaLEP0199285. May amplify unspliced transcripts of an antisense gene.

List Price:    $174.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   lncRNA Expression
Unique Assay ID:   qhsaLED0122045
Assay Design:   exonic
Chromosome Location:   chr10:88992020-88992124question
Amplicon Length:   75
LNCipedia Splice Variants Targeted:   lnc-ACTA2-1:1 lnc-ACTA2-1:2 lnc-ACTA2-1:3

Gene Information

Not Available

Gene Symbol:   lnc-ACTA2-1
Gene Name:   Not Available
Aliases:   SAF, FAS-AS (FAS antisense RNA)
RefSeq:   Not Available
LNCipedia:   lnc-ACTA2-1
Chromosome Mapping:   Not Available
Gene Strand:   Reverse strand

The below validation information is for the Primer Pair only  


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.950001
Efficiency 1.97

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download