PrimePCR™ lncRNA SYBR® Green Assay:CCDC125, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression.

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qhsaLEP0211915

List Price:    $169.00
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PrimePCR™ IncRNA PreAmp for SYBR® Green Assay: CCDC125, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ IncRNA Template for SYBR® Green Assay: CCDC125, Human
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   lncRNA Expression
Unique Assay ID:   qhsaLED0134675
Assay Design:   exonic
Chromosome Location:   chr5:69320266-69320371question
Amplicon Length:   76
Splice Variants Targeted:   ENST00000511257 ENST00000383374 ENST00000460090 ENST00000396496 ENST00000396499 ENST00000513172 ENST00000512045
LNCipedia Splice Variants Targeted:   lnc-TAF9-3:3 lnc-TAF9-3:2 lnc-TAF9-3:1

Gene Information

Description Not Available

Gene Symbol:   CCDC125
Gene Name:   coiled-coil domain containing 125
Aliases:   KENAE
RefSeq:   NC_000005.9 NT_006713.15 NW_003315917.1
Ensembl:   ENSG00000183323
LNCipedia:   lnc-TAF9-3
Entrez:   202243
Chromosome Mapping:   5q13.2
Gene Strand:   Not Available

The below validation information is for the Primer Pair only  

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 35.490002
Efficiency 1.99

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download