PrimePCR™ lncRNA SYBR® Green Assay:LINC00847, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression.

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qhsaLEP0211991. May amplify unspliced transcripts of an antisense gene.

List Price:    $174.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   lncRNA Expression
Unique Assay ID:   qhsaLED0134751
Assay Design:   exonic
Chromosome Location:   chr5:180834621-180834787question
Amplicon Length:   137
Refseq Transcripts Amplified:   NR_027183.2,NR_045678.1,NR_045679.1
Splice Variants Targeted:   ENST00000501855 ENST00000502162 ENST00000509921 ENST00000501937 ENST00000508309
LNCipedia Splice Variants Targeted:   LINC00847:2 LINC00847:7 LINC00847:3 LINC00847:4 LINC00847:11 LINC00847:13 LINC00847:15 LINC00847:16 LINC00847:14 LINC00847:12 LINC00847:10 LINC00847:5 LINC00847:8 LINC00847:6

Gene Information

Not Available

Gene Symbol:   LINC00847
Gene Name:   long intergenic non-protein coding RNA 847 [Source:HGNC Symbol;Acc:HGNC:45050]
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSG00000245060
LNCipedia:   LINC00847
Entrez:   729678
Chromosome Mapping:   5q35.3
Gene Strand:   Forward strand

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 35.630001
Efficiency 1.99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download