PrimePCR™ lncRNA SYBR® Green Assay:DLEU1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression.

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qhsaLEP0212109

List Price:    $169.00
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PrimePCR™ IncRNA PreAmp for SYBR® Green Assay: DLEU1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ IncRNA Template for SYBR® Green Assay: DLEU1, Human
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   lncRNA Expression
Unique Assay ID:   qhsaLED0134869
Assay Design:   exonic
Chromosome Location:   chr13:50083185-50415170question
Amplicon Length:   62
Splice Variants Targeted:   ENST00000470593 ENST00000460525 ENST00000498557 ENST00000462427 ENST00000484869 ENST00000470726 ENST00000491482 ENST00000474630 ENST00000461527 ENST00000463474 ENST00000476738 ENST00000467721 ENST00000490577 ENST00000491615 ENST00000468522 ENST00000491341 ENST00000468168 ENST00000485007 ENST00000475913 ENST00000469127 ENST00000479420 ENST00000480262 ENST00000469095 ENST00000472136
LNCipedia Splice Variants Targeted:   DLEU1:15 DLEU1:14 DLEU1:18 DLEU1:6 DLEU1:12 DLEU1:8 DLEU1:51 DLEU1:40 DLEU1:36 DLEU1:17 DLEU1:48 DLEU1:37 DLEU1:44 DLEU1:39 DLEU1:27 DLEU1:38 DLEU1:50 DLEU1:7 DLEU1:20 DLEU1:19 DLEU1:13 DLEU1:35 DLEU1:31 DLEU1:10 DLEU1:11 DLEU1:21 DLEU1:1 DLEU1:47 DLEU1:32 DLEU1:45 DLEU1:42 DLEU1:34 DLEU1:33 DLEU1:49 DLEU1:46 DLEU1:30 DLEU1:43

Gene Information

Description Not Available

Gene Symbol:   DLEU1
Gene Name:   deleted in lymphocytic leukemia 1 (non-protein coding)
Aliases:   BCMS, DLB1, DLEU2, LEU1, LEU2, MGC22430, NCRNA00021, XTP6
RefSeq:   NC_000013.10 NT_024524.14
Ensembl:   ENSG00000176124
LNCipedia:   DLEU1
Entrez:   10301
Chromosome Mapping:   13q14.3
Gene Strand:   Not Available

The below validation information is for the Primer Pair only  

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998800
y-intercept 35.459999
Efficiency 2

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download