PrimePCR™ lncRNA SYBR® Green Assay:KRT81, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression.

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qhsaLEP0212150. May amplify unspliced transcripts of an antisense gene.

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PrimePCR™ IncRNA PreAmp for SYBR® Green Assay: KRT81, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ IncRNA Template for SYBR® Green Assay: KRT81, Human
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   lncRNA Expression
Unique Assay ID:   qhsaLED0134910
Assay Design:   exonic
Chromosome Location:   chr12:52286040-52286156question
Amplicon Length:   87
Splice Variants Targeted:   ENST00000327741
LNCipedia Splice Variants Targeted:   lnc-KRT81-1:1 lnc-KRT81-1:2

Gene Information

The protein encoded by this gene is a member of the keratin gene family. As a type II hair keratin it is a basic protein which heterodimerizes with type I keratins to form hair and nails. The type II hair keratins are clustered in a region of chromosome 12q13 and are grouped into two distinct subfamilies based on structure similarity. One subfamily consisting of KRTHB1 KRTHB3 and KRTHB6 is highly related. The other less-related subfamily includes KRTHB2 KRTHB4 and KRTHB5. All hair keratins are expressed in the hair follicle; this hair keratin as well as KRTHB3 and KRTHB6 is found primarily in the hair cortex. Mutations in this gene and KRTHB6 have been observed in patients with a rare dominant hair disease monilethrix. [provided by RefSeq Jul 2008]

Gene Symbol:   KRT81
Gene Name:   keratin 81
Aliases:   HB1, Hb-1, KRTHB1, MLN137, ghHkb1, hHAKB2-1
RefSeq:   NC_000012.11 NG_008184.1 NT_029419.12
Ensembl:   ENSG00000205426
LNCipedia:   lnc-KRT81-1
Entrez:   3887
Chromosome Mapping:   12q13
Gene Strand:   Not Available

The below validation information is for the Primer Pair only  

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997900
y-intercept 35.259998
Efficiency 2.06

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download