PrimePCR™ lncRNA SYBR® Green Assay:NDUFA1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression.

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qhsaLIP0181674

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PrimePCR™ IncRNA PreAmp for SYBR® Green Assay: NDUFA1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ IncRNA Template for SYBR® Green Assay: NDUFA1, Human
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   lncRNA Expression
Unique Assay ID:   qhsaLID0104434
Assay Design:   intron_spanning
Chromosome Location:   chrX:119871948-119876594question
Amplicon Length:   117
Refseq Transcripts Amplified:   NM_004541
Splice Variants Targeted:   ENST00000371437
LNCipedia Splice Variants Targeted:   lnc-NDUFA1-1:1

Gene Information

The human NDUFA1 gene codes for an essential component of complex I of the respiratory chain which transfers electrons from NADH to ubiquinone. It has been noted that the N-terminal hydrophobic domain has the potential to be folded into an alpha-helix spanning the inner mitochondrial membrane with a C-terminal hydrophilic domain interacting with globular subunits of complex I. The highly conserved two-domain structure suggests that this feature is critical for the protein function and might act as an anchor for the NADH:ubiquinone oxidoreductase complex at the inner mitochondrial membrane. However the NDUFA1 peptide is one of about 31 components of the "hydrophobic protein" (HP) fraction of complex I which is involved in proton translocation. Thus the NDUFA1 peptide may also participate in that function. [provided by RefSeq Jul 2008]

Gene Symbol:   NDUFA1
Gene Name:   NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 1, 7.5kDa
Aliases:   CI-MWFE, MWFE, ZNF183
RefSeq:   NC_000023.10 NG_009381.1 NG_021227.1 NT_011786.16
Ensembl:   ENSG00000125356
LNCipedia:   lnc-NDUFA1-1
Entrez:   4694
Chromosome Mapping:   Xq24
Gene Strand:   Not Available

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10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download