PrimePCR™ lncRNA SYBR® Green Assay:AFAP1-AS1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression.

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info: Same primer pair as used in probe assay qhsaLIP0211933


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PrimePCR™ IncRNA PreAmp for SYBR® Green Assay: AFAP1-AS1, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ IncRNA Template for SYBR® Green Assay: AFAP1-AS1, Human
Reaction: 4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		lncRNA Expression
Unique Assay ID:		qhsaLID0134693
Assay Design:		intron_spanning
Chromosome Location:		chr4:7754093-7772319question
Amplicon Length:		153
Refseq Transcripts Amplified:		NR_026892.1
Splice Variants Targeted:		ENST00000608442
LNCipedia Splice Variants Targeted:		lnc-SORCS2-1:2 lnc-SORCS2-1:1 lnc-SORCS2-1:3

Gene Information

Not Available

Gene Symbol:		AFAP1-AS1
Gene Name:		AFAP1 antisense RNA 1 [Source:HGNC Symbol;Acc:HGNC:28141]
Aliases:		AFAP1-AS, AFAP1AS
RefSeq:		Not Available
Ensembl:		ENSG00000272620
LNCipedia:		lnc-SORCS2-1
Entrez:		84740
Chromosome Mapping:		4p16.1
Gene Strand:		Forward strand

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.995000
y-intercept	34.970001
Efficiency	2.04

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.