PrimePCR™ SYBR® Green Assay: Oxt, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qMmuCEP0034766


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PrimePCR™ PreAmp for SYBR® Green Assay: Oxt, Mouse
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: Oxt, Mouse
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qMmuCED0004477
Assay Design:		Exonic
Chromosome Location:		2:130576890-130576980question
Amplicon Length:		61
Splice Variants Targeted:		ENSMUST00000167994 ENSMUST00000025288 ENSMUST00000098250 ENSMUST00000032931 ENSMUST00000145555 ENSMUST00000044188 ENSMUST00000098312 ENSMUST00000030676 ENSMUST00000029814 ENSMUST00000109937 ENSMUST00000109936 ENSMUST00000040455 ENSMUST00000159610 ENSMUST00000159048 ENSMUST00000078496 ENSMUST00000072141 ENSMUST00000028764 ENSMUST00000120691 ENSMUST00000043338 ENSMUST00000082337

Gene Information

Description Not Available

Gene Symbol:		Oxt
Gene Name:		Oxytocin
Aliases:		OT, Oxy
RefSeq:		NC_000068.7 NT_039207.8
Ensembl:		ENSMUSG00000027301
Entrez:		18429
Chromosome Mapping:		2 F1\|2 73.5 cM

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Mouse Reference Total RNA

Summary Data:

R²	0.999500
y-intercept	35.680000
Efficiency	95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.