PrimePCR™ SYBR® Green Assay: Zfp345, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best negative control

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Zfp345, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Zfp345, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0004863
Assay Design:   Exonic
Chromosome Location:   2:150471104-150471250question
Amplicon Length:   117
Splice Variants Targeted:   ENSMUST00000033739 ENSMUST00000033621 ENSMUST00000056470 ENSMUST00000016511 ENSMUST00000109914 ENSMUST00000029445 ENSMUST00000032469 ENSMUST00000112691 ENSMUST00000026297 ENSMUST00000130451 ENSMUST00000122424 ENSMUST00000086153 ENSMUST00000105608 ENSMUST00000142911 ENSMUST00000148287 ENSMUST00000035056 ENSMUST00000136969 ENSMUST00000149251 ENSMUST00000138940 ENSMUST00000144180 ENSMUST00000149199

Gene Information

Description Not Available

Gene Symbol:   Zfp345
Gene Name:   Zinc finger protein 345
Aliases:   OTTMUSG00000015743
RefSeq:   NC_000068.7 NT_039207.8
Ensembl:   ENSMUSG00000074731
Entrez:   545471
Chromosome Mapping:   2 G3|2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.070000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download