PrimePCR™ SYBR® Green Assay: Sphk1, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qMmuCEP0042692


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PrimePCR™ PreAmp for SYBR® Green Assay: Sphk1, Mouse
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: Sphk1, Mouse
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qMmuCED0040475
Assay Design:		exonic
Chromosome Location:		11:116535088-116535256question
Amplicon Length:		61
Splice Variants Targeted:		ENSMUST00000106388 ENSMUST00000138840 ENSMUST00000106387 ENSMUST00000100201 ENSMUST00000106386 ENSMUST00000145737 ENSMUST00000155102 ENSMUST00000063446 ENSMUST00000063396 ENSMUST00000154034 ENSMUST00000124682 ENSMUST00000141798

Gene Information

This gene encodes a kinase that phosphorylates sphingosine into sphingosine-1-phosphate which is involved in cell differentiation motility and apoptosis. The encoded protein plays a role in maintaining cellular levels of sphingosine-1-phosphate. Alternative splicing results in multiple transcript variants. [provided by RefSeq Feb 2010]

Gene Symbol:		Sphk1
Gene Name:		Sphingosine kinase 1
Aliases:		1110006G24Rik, Sk1, Spk1
RefSeq:		NC_000077.6 NT_096135.6
Ensembl:		ENSMUSG00000061878
Entrez:		20698
Chromosome Mapping:		11 E2\|11

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Mouse Reference Total RNA

Summary Data:

R²	0.999300
y-intercept	35.750000
Efficiency	99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.