PrimePCR™ SYBR® Green Assay: Ins2, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCEP0042993

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Ins2, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Ins2, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0040776
Assay Design:   exonic
Chromosome Location:   7:142678710-142678823question
Amplicon Length:   84
Splice Variants Targeted:   ENSMUST00000002964 ENSMUST00000075843 ENSMUST00000109802 ENSMUST00000166939 ENSMUST00000027475 ENSMUST00000172794 ENSMUST00000164992 ENSMUST00000173173 ENSMUST00000174501 ENSMUST00000105931 ENSMUST00000105934 ENSMUST00000105930 ENSMUST00000105933 ENSMUST00000000220 ENSMUST00000105932 ENSMUST00000162317 ENSMUST00000085803 ENSMUST00000115487 ENSMUST00000039652 ENSMUST00000001059 ENSMUST00000140920 ENSMUST00000030862 ENSMUST00000037434 ENSMUST00000103027 ENSMUST00000177138 ENSMUST00000108617 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Ins2
Gene Name:   Insulin II
Aliases:   AA986540, Ins-2, InsII, Mody, Mody4, proinsulin
RefSeq:   NC_000073.6 NT_039437.8
Ensembl:   ENSMUSG00000000215
Entrez:   16334
Chromosome Mapping:   7 F5|7 69.1 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 41.940000
Efficiency 80

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download