PrimePCR™ SYBR® Green Assay: Top3b, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qMmuCEP0043037


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PrimePCR™ PreAmp for SYBR® Green Assay: Top3b, Mouse
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: Top3b, Mouse
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qMmuCED0040820
Assay Design:		exonic
Chromosome Location:		16:16877832-16877931question
Amplicon Length:		70
Splice Variants Targeted:		ENSMUST00000139740 ENSMUST00000119787 ENSMUST00000130650 ENSMUST00000156502 ENSMUST00000023465 ENSMUST00000124960 ENSMUST00000144513 ENSMUST00000131063 ENSMUST00000163799 ENSMUST00000032927 ENSMUST00000172630 ENSMUST00000174291 ENSMUST00000164479

Gene Information

Description Not Available

Gene Symbol:		Top3b
Gene Name:		Topoisomerase (DNA) III beta
Aliases:		Not Available
RefSeq:		NC_000082.6 NT_039624.8
Ensembl:		ENSMUSG00000022779
Entrez:		21976
Chromosome Mapping:		16\|16 B1

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Mouse Reference Total RNA

Summary Data:

R²	0.999800
y-intercept	37.250000
Efficiency	91

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.