PrimePCR™ SYBR® Green Assay: Mobp, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCEP0043101

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Mobp, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Mobp, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0040884
Assay Design:   exonic
Chromosome Location:   9:120167882-120167976question
Amplicon Length:   65
Splice Variants Targeted:   ENSMUST00000113800 ENSMUST00000099497 ENSMUST00000019276 ENSMUST00000023805 ENSMUST00000120122 ENSMUST00000034529 ENSMUST00000166367 ENSMUST00000042485 ENSMUST00000119722 ENSMUST00000143031 ENSMUST00000044217 ENSMUST00000033611 ENSMUST00000034090 ENSMUST00000084086 ENSMUST00000090190 ENSMUST00000115698 ENSMUST00000006053 ENSMUST00000163476 ENSMUST00000165363 ENSMUST00000090159 ENSMUST00000170996 ENSMUST00000170366 ENSMUST00000163997 ENSMUST00000170273 ENSMUST00000108408 ENSMUST00000112986 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Mobp
Gene Name:   Myelin-associated oligodendrocytic basic protein
Aliases:   AI835053, AI839848, MOBP155, MOBP170, MOBP69, MOBP73, MOBP81, R74645
RefSeq:   NC_000075.6 NT_039482.8
Ensembl:   ENSMUSG00000032517
Entrez:   17433
Chromosome Mapping:   9 F4|9 69.0 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 36.860000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download