PrimePCR™ SYBR® Green Assay: Try5, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qMmuCEP0043121

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PrimePCR™ PreAmp for SYBR® Green Assay: Try5, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: Try5, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0040904
Assay Design:   exonic
Chromosome Location:   6:41311288-41311717question
Amplicon Length:   133
Splice Variants Targeted:   ENSMUST00000092415 ENSMUST00000108709 ENSMUST00000120204 ENSMUST00000106017 ENSMUST00000121049 ENSMUST00000106015 ENSMUST00000106018 ENSMUST00000097868 ENSMUST00000030571 ENSMUST00000030136 ENSMUST00000034865 ENSMUST00000076638 ENSMUST00000166306 ENSMUST00000031931 ENSMUST00000031913 ENSMUST00000064324 ENSMUST00000173916 ENSMUST00000072103 ENSMUST00000096003 ENSMUST00000049079 ENSMUST00000095999 ENSMUST00000031910 ENSMUST00000070380 ENSMUST00000051482 ENSMUST00000001974 ENSMUST00000109767 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Try5
Gene Name:   Trypsin 5
Aliases:   1810049H19Rik, Tc, Tesp4
RefSeq:   NC_000072.6 NG_006980.1 NT_039353.8
Ensembl:   ENSMUSG00000036938
Entrez:   103964
Chromosome Mapping:   6 B1|6

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 36.900000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download