PrimePCR™ SYBR® Green Assay: Trex1, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best specificity; Same primer pair as used in probe assay qMmuCEP0043188

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Trex1, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Trex1, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0040971
Assay Design:   exonic
Chromosome Location:   9:109058523-109058672question
Amplicon Length:   120
Splice Variants Targeted:   ENSMUST00000023631 ENSMUST00000113829 ENSMUST00000153398 ENSMUST00000099502 ENSMUST00000061973 ENSMUST00000112053 ENSMUST00000128062 ENSMUST00000119888 ENSMUST00000053273 ENSMUST00000079573 ENSMUST00000043983 ENSMUST00000007280

Gene Information

Description Not Available

Gene Symbol:   Trex1
Gene Name:   Three prime repair exonuclease 1
Aliases:   1661, AU041952
RefSeq:   NC_000075.6 NT_039482.8
Ensembl:   ENSMUSG00000049734
Entrez:   22040
Chromosome Mapping:   9 F2|9

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 38.040000
Efficiency 92

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download