PrimePCR™ SYBR® Green Assay: Rpl11, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Recommended - best specificity; Same primer pair as used in probe assay qMmuCEP0043288


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PrimePCR™ PreAmp for SYBR® Green Assay: Rpl11, Mouse
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: Rpl11, Mouse
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qMmuCED0041071
Assay Design:		exonic
Chromosome Location:		4:136052567-136052657question
Amplicon Length:		61
Splice Variants Targeted:		ENSMUST00000162812 ENSMUST00000161273 ENSMUST00000161938 ENSMUST00000086123 ENSMUST00000022386 ENSMUST00000125113 ENSMUST00000026698 ENSMUST00000024958 ENSMUST00000039926 ENSMUST00000037646 ENSMUST00000057037 ENSMUST00000079733 ENSMUST00000150948 ENSMUST00000059886 ENSMUST00000051377 ENSMUST00000058844 ENSMUST00000077093 ENSMUST00000023722 ENSMUST00000169721 ENSMUST00000156290 ENSMUST00000040111 ENSMUST00000171075 ENSMUST00000108372 ENSMUST00000166852 ENSMUST00000035083 ENSMUST00000178048 ENSMUS

Gene Information

Description Not Available

Gene Symbol:		Rpl11
Gene Name:		Ribosomal protein L11
Aliases:		2010203J19Rik
RefSeq:		NC_000070.6 NT_187033.1
Ensembl:		ENSMUSG00000059291
Entrez:		67025
Chromosome Mapping:		4 D3\|4

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Mouse Reference Total RNA

Summary Data:

R²	0.999800
y-intercept	36.680000
Efficiency	97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.