PrimePCR™ SYBR® Green Assay: Hist2h2aa1, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCEP0043389

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Hist2h2aa1, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Hist2h2aa1, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0041172
Assay Design:   exonic
Chromosome Location:   3:96245540-96245635question
Amplicon Length:   66
Splice Variants Targeted:   ENSMUST00000027405 ENSMUST00000040359 ENSMUST00000108387 ENSMUST00000153452 ENSMUST00000122040 ENSMUST00000117252 ENSMUST00000071780 ENSMUST00000076737 ENSMUST00000109158 ENSMUST00000069922 ENSMUST00000022589 ENSMUST00000008733 ENSMUST00000039840 ENSMUST00000119686 ENSMUST00000161069 ENSMUST00000022691 ENSMUST00000163060 ENSMUST00000134863 ENSMUST00000073364 ENSMUST00000123434 ENSMUST00000146396 ENSMUST00000161516 ENSMUST00000099739 ENSMUST00000042035 ENSMUST00000170845 ENSMUST00000078756 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Hist2h2aa1
Gene Name:   Histone cluster 2, H2aa1
Aliases:   AI448581, H2a-615, Hist2
RefSeq:   NC_000069.6 NT_039240.8
Ensembl:   ENSMUSG00000063954
Entrez:   15267
Chromosome Mapping:   3 F1-F2|3 45.2 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 36.080000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download