PrimePCR™ SYBR® Green Assay: Defa3, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCEP0043575

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Defa3, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Defa3, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0041359
Assay Design:   exonic
Chromosome Location:   8:21287409-21287586question
Amplicon Length:   148
Splice Variants Targeted:   ENSMUST00000136925 ENSMUST00000061673 ENSMUST00000023826 ENSMUST00000108288 ENSMUST00000055110 ENSMUST00000110758 ENSMUST00000076754 ENSMUST00000098899 ENSMUST00000078121 ENSMUST00000098897 ENSMUST00000110754 ENSMUST00000098893 ENSMUST00000098890 ENSMUST00000120874 ENSMUST00000110752 ENSMUST00000084042 ENSMUST00000084041 ENSMUST00000084040 ENSMUST00000110749 ENSMUST00000071886 ENSMUST00000080533 ENSMUST00000022622 ENSMUST00000089250 ENSMUST00000111121 ENSMUST00000178730 ENSMUST00000086615 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Defa3
Gene Name:   Defensin, alpha, 3
Aliases:   Defa27, Defcr-3, Defcr3
RefSeq:   NC_000074.6 NT_039457.8
Ensembl:   ENSMUSG00000074440
Entrez:   13237
Chromosome Mapping:   8 A2|8

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.998700
y-intercept 39.280000
Efficiency 90

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download