PrimePCR™ SYBR® Green Assay: Mef2b, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCEP0043655

List Price:    $174.00
Your Price:   Log In
Quantity:  
 
Add to Custom Plate

PrimePCR™ PreAmp for SYBR® Green Assay: Mef2b, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Mef2b, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0041439
Assay Design:   exonic
Chromosome Location:   8:70167165-70167305question
Amplicon Length:   111
Splice Variants Targeted:   ENSMUST00000058092 ENSMUST00000027940 ENSMUST00000072940 ENSMUST00000093162 ENSMUST00000114571 ENSMUST00000031815 ENSMUST00000077093 ENSMUST00000114572 ENSMUST00000113658 ENSMUST00000102944 ENSMUST00000032279 ENSMUST00000041195 ENSMUST00000036618 ENSMUST00000163756 ENSMUST00000072500 ENSMUST00000164040 ENSMUST00000110146 ENSMUST00000110143 ENSMUST00000110141 ENSMUST00000110140

Gene Information

Description Not Available

Gene Symbol:   Mef2b
Gene Name:   Myocyte enhancer factor 2B
Aliases:   AI451606
RefSeq:   NC_000074.6 NT_039462.8
Ensembl:   ENSMUSG00000079033
Entrez:   17259
Chromosome Mapping:   8 C1|8 33.5 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.995700
y-intercept 39.090000
Efficiency 86

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download