PrimePCR™ SYBR® Green Assay: Rhox2h, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best specificity; Same primer pair as used in probe assay qMmuCEP0043672

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PrimePCR™ PreAmp for SYBR® Green Assay: Rhox2h, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: Rhox2h, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0041456
Assay Design:   exonic
Chromosome Location:   X:37672703-37672897question
Amplicon Length:   165
Splice Variants Targeted:   ENSMUST00000063340 ENSMUST00000173650 ENSMUST00000115191 ENSMUST00000173802 ENSMUST00000115190 ENSMUST00000173574 ENSMUST00000115179 ENSMUST00000072167 ENSMUST00000115173 ENSMUST00000115169 ENSMUST00000115162

Gene Information

Description Not Available

Gene Symbol:   Rhox2h
Gene Name:   Reproductive homeobox 2H
Aliases:   OTTMUSG00000017156, Rhox2.8, Rhox2g
RefSeq:   NC_000086.7 NT_187037.1
Ensembl:   ENSMUSG00000079627
Entrez:   622301
Chromosome Mapping:   X A3.3|X

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 39.210000
Efficiency 86

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download