PrimePCR™ SYBR® Green Assay: Flt3l, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCEP0043697

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Flt3l, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Flt3l, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0041481
Assay Design:   exonic
Chromosome Location:   7:45133902-45134020question
Amplicon Length:   89
Splice Variants Targeted:   ENSMUST00000052164 ENSMUST00000041674 ENSMUST00000045736 ENSMUST00000037615 ENSMUST00000128119 ENSMUST00000085901 ENSMUST00000172467 ENSMUST00000093162 ENSMUST00000124300 ENSMUST00000085377 ENSMUST00000146760 ENSMUST00000125500 ENSMUST00000130628 ENSMUST00000035471 ENSMUST00000032715 ENSMUST00000049308 ENSMUST00000015100 ENSMUST00000090511 ENSMUST00000064377 ENSMUST00000107942 ENSMUST00000173206 ENSMUST00000172288 ENSMUST00000111764 ENSMUST00000093527 ENSMUST00000087517 ENSMUST00000094218 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Flt3l
Gene Name:   FMS-like tyrosine kinase 3 ligand
Aliases:   Flt3lg, Ly72L
RefSeq:   NC_000073.6 NT_187035.1
Ensembl:   ENSMUSG00000089989
Entrez:   14256
Chromosome Mapping:   7 B2-C|7 23.0 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 37.310000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download