PrimePCR™ SYBR® Green Assay: CT573034.1, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCEP0043864

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PrimePCR™ PreAmp for SYBR® Green Assay: CT573034.1, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: CT573034.1, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0041648
Assay Design:   exonic
Chromosome Location:   13:62371814-62371912question
Amplicon Length:   69
Splice Variants Targeted:   ENSMUST00000122450 ENSMUST00000113729 ENSMUST00000170101 ENSMUST00000034259 ENSMUST00000105315 ENSMUST00000105316 ENSMUST00000099439 ENSMUST00000076281 ENSMUST00000105313 ENSMUST00000099449 ENSMUST00000107989 ENSMUST00000076195 ENSMUST00000101547 ENSMUST00000091563 ENSMUST00000167516 ENSMUST00000037372 ENSMUST00000089217 ENSMUST00000001989 ENSMUST00000025645 ENSMUST00000120524 ENSMUST00000130783 ENSMUST00000144085 ENSMUST00000048892 ENSMUST00000105718 ENSMUST00000135798 ENSMUST00000112547 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   CT573034.1
Gene Name:   Uncharacterized protein LOC100041979
Aliases:   Not Available
RefSeq:   NC_000079.6 NT_039589.8
Ensembl:   ENSMUSG00000094942
Entrez:   100041979
Chromosome Mapping:   13 B3|13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 40.470000
Efficiency 84

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download