PrimePCR™ SYBR® Green Assay: Dio2, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCEP0052679

List Price:    $179.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Dio2, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Dio2, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0044253
Assay Design:   exonic
Chromosome Location:   12:90726289-90726434question
Amplicon Length:   116
Splice Variants Targeted:   ENSMUST00000082432

Gene Information

The protein encoded by this gene belongs to the iodothyronine deiodinase family. It activates thyroid hormone by converting the prohormone thyroxine (T4) by outer ring deiodination (ORD) to bioactive 33'5-triiodothyronine (T3). Knockout studies in mice suggest that this gene may play a role in brown adipose tissue lipogenesis auditory function and bone formation. This protein contains selenocysteine (Sec) residues encoded by the UGA codon which normally signals translation termination. The 3' UTR of Sec-containing genes have a common stem-loop structure the sec insertion sequence (SECIS) which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. [provided by RefSeq Jul 2008]

Gene Symbol:   Dio2
Gene Name:   Deiodinase, iodothyronine, type II
Aliases:   5DII, AI324267, DIOII
RefSeq:   NC_000078.6 NT_039551.8
Ensembl:   ENSMUSG00000007682
Entrez:   13371
Chromosome Mapping:   12 D3|12

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