PrimePCR™ SYBR® Green Assay: Dhtkd1, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qMmuCEP0054054

List Price:    $146.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0045628
Assay Design:   exonic
Chromosome Location:   2:5919485-5919632question
Amplicon Length:   118
Splice Variants Targeted:   ENSMUST00000095147 ENSMUST00000026924 ENSMUST00000169865

Gene Information

Description Not Available

Gene Symbol:   Dhtkd1
Gene Name:   Dehydrogenase E1 and transketolase domain containing 1
Aliases:   C330018I04Rik
RefSeq:   NC_000068.7 NT_039202.8
Ensembl:   ENSMUSG00000025815
Entrez:   209692
Chromosome Mapping:   2 A1|2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.997300
y-intercept 37.200000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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