PrimePCR™ SYBR® Green Assay: Nfasc, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Recommended - best coverage; Same primer pair as used in probe assay qMmuCEP0054296


List Price:		$179.00
Your Price:		Log In
Quantity:		Add to Cart Add to Cart
Add to My PrimePCR Add to My PrimePCR		Add to Quote Add to Quote
Add to Custom Plate

PrimePCR™ PreAmp for SYBR® Green Assay: Nfasc, Mouse
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

List Price:		$174.00
Your Price:		Log In
Quantity:		Add to Cart Add to Cart
[ Add to My PrimePCR ] [ Add to My PrimePCR ]		[ Add to Quote ] [ Add to Quote ]

PrimePCR™ Template for SYBR® Green Assay: Nfasc, Mouse
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

List Price:		$174.00
Your Price:		Log In
Quantity:		Add to Cart Add to Cart
[ Add to My PrimePCR ] [ Add to My PrimePCR ]		[ Add to Quote ] [ Add to Quote ]

Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qMmuCED0045870
Assay Design:		exonic
Chromosome Location:		1:132569511-132569621question
Amplicon Length:		81
Splice Variants Targeted:		ENSMUST00000094569 ENSMUST00000163770 ENSMUST00000043189

Gene Information

This gene encodes an L1 family immunoglobulin cell adhesion molecule with multiple IGcam and fibronectin domains. The protein functions in neurite outgrowth neurite fasciculation and organization of the axon initial segment (AIS) and nodes of Ranvier on axons during early development. Both the AIS and nodes of Ranvier contain high densities of voltage-gated Na+ (Nav) channels which are clustered by interactions with cytoskeletal and scaffolding proteins including this protein gliomedin ankyrin 3 (ankyrin-G) and betaIV spectrin. This protein links the AIS extracellular matrix to the intracellular cytoskeleton. This gene undergoes extensive alternative splicing and the full-length nature of some variants has not been determined. [provided by RefSeq May 2009]

Gene Symbol:		Nfasc
Gene Name:		Neurofascin
Aliases:		AA387016, D430023G06Rik, NF, mKIAA0756
RefSeq:		NC_000067.6 NT_078297.7
Ensembl:		ENSMUSG00000026442
Entrez:		269116
Chromosome Mapping:		1 E4\|1 70.0 cM

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Mouse Reference Total RNA

Summary Data:

R²	0.999400
y-intercept	34.750000
Efficiency	104

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.