PrimePCR™ SYBR® Green Assay: Mcfd2, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qMmuCEP0055217

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Mcfd2, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Mcfd2, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0046791
Assay Design:   exonic
Chromosome Location:   17:87258001-87264157question
Amplicon Length:   76
Splice Variants Targeted:   ENSMUST00000034905 ENSMUST00000072093 ENSMUST00000114884 ENSMUST00000059644 ENSMUST00000024963 ENSMUST00000144236 ENSMUST00000129616 ENSMUST00000151155 ENSMUST00000145895 ENSMUST00000155904 ENSMUST00000030980 ENSMUST00000162645 ENSMUST00000007961 ENSMUST00000057799 ENSMUST00000052904 ENSMUST00000061099 ENSMUST00000036618 ENSMUST00000159249 ENSMUST00000021311

Gene Information

Description Not Available

Gene Symbol:   Mcfd2
Gene Name:   Multiple coagulation factor deficiency 2
Aliases:   1810021C21Rik, F5f8d, Lman1ip, Sdnsf
RefSeq:   NC_000083.6 NT_039649.8
Ensembl:   ENSMUSG00000024150
Entrez:   193813
Chromosome Mapping:   17 E4|17

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.610000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download