PrimePCR™ SYBR® Green Assay: Smarcc1, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qMmuCEP0055824

List Price:    $146.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0047398
Assay Design:   exonic
Chromosome Location:   9:110171063-110173994question
Amplicon Length:   89
Splice Variants Targeted:   ENSMUST00000088716 ENSMUST00000111969 ENSMUST00000035057 ENSMUST00000111966 ENSMUST00000111968

Gene Information

Description Not Available

Gene Symbol:   Smarcc1
Gene Name:   SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily c, member 1
Aliases:   AI115498, BAF155, Rsc8, SRG3
RefSeq:   NC_000075.6 NT_039482.8
Ensembl:   ENSMUSG00000032481
Entrez:   20588
Chromosome Mapping:   9 F2|9

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.530000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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