PrimePCR™ SYBR® Green Assay: Arpp21, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCEP0056581

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Arpp21, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Arpp21, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0048155
Assay Design:   exonic
Chromosome Location:   9:112183516-112184298question
Amplicon Length:   69
Splice Variants Targeted:   ENSMUST00000178958 ENSMUST00000058725 ENSMUST00000057258 ENSMUST00000179555 ENSMUST00000043503 ENSMUST00000105359 ENSMUST00000020349 ENSMUST00000018184 ENSMUST00000130053 ENSMUST00000122985 ENSMUST00000127380 ENSMUST00000139581 ENSMUST00000093785 ENSMUST00000035202 ENSMUST00000107876 ENSMUST00000003044 ENSMUST00000098478 ENSMUST00000021459 ENSMUST00000102491 ENSMUST00000097816 ENSMUST00000168157 ENSMUST00000040222 ENSMUST00000023750 ENSMUST00000033761 ENSMUST00000128136 ENSMUST00000114372 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Arpp21
Gene Name:   Cyclic AMP-regulated phosphoprotein, 21
Aliases:   0710001E13Rik, AI853636, ARPP-21, D9Bwg1012e, Tarpp
RefSeq:   NC_000075.6 NT_039482.8
Ensembl:   ENSMUSG00000032503
Entrez:   74100
Chromosome Mapping:   9 F3|9 59.0 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.470000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download