PrimePCR™ SYBR® Green Assay: Fxyd1, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qMmuCEP0057940

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Fxyd1, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Fxyd1, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0049514
Assay Design:   exonic
Chromosome Location:   7:31051698-31051787question
Amplicon Length:   60
Splice Variants Targeted:   ENSMUST00000006893 ENSMUST00000039909 ENSMUST00000071697 ENSMUST00000108110 ENSMUST00000105856

Gene Information

This gene encodes a member of the FXYD family of small membrane proteins that share a 35-amino acid signature sequence domain beginning with the sequence PFXYD and containing 7 invariant and 6 highly conserved amino acids. The protein encoded by this gene is a plasma membrane substrate for several kinases including protein kinase A protein kinase C NIMA kinase and myotonic dystrophy kinase. It is thought to form an ion channel or regulate ion channel activity and act as an accessory protein of NaK-ATPase. Alternatively spliced transcript variants have been described. [provided by RefSeq Sep 2009]

Gene Symbol:   Fxyd1
Gene Name:   FXYD domain-containing ion transport regulator 1
Aliases:   0610012C17Rik, 1110006M24Rik, Plm, Pml
RefSeq:   NC_000073.6 NT_187034.1
Ensembl:   ENSMUSG00000036570
Entrez:   56188
Chromosome Mapping:   7|7 B1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 36.040000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download