PrimePCR™ SYBR® Green Assay: Tmed5, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qMmuCEP0060581

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Tmed5, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Tmed5, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0052156
Assay Design:   exonic
Chromosome Location:   5:108132317-108132469question
Amplicon Length:   123
Splice Variants Targeted:   ENSMUST00000165766 ENSMUST00000173397 ENSMUST00000072362 ENSMUST00000091087 ENSMUST00000172482 ENSMUST00000174050 ENSMUST00000115404 ENSMUST00000114943 ENSMUST00000073379 ENSMUST00000034851 ENSMUST00000022629 ENSMUST00000107975 ENSMUST00000107976 ENSMUST00000069184 ENSMUST00000078680 ENSMUST00000009667 ENSMUST00000154292 ENSMUST00000177212 ENSMUST00000176371 ENSMUST00000118036 ENSMUST00000061203 ENSMUST00000002837 ENSMUST00000119437 ENSMUST00000117759 ENSMUST00000119784 ENSMUST00000068904 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Tmed5
Gene Name:   Transmembrane emp24 protein transport domain containing 5
Aliases:   3110020O18Rik, 4432412D15Rik
RefSeq:   NC_000071.6 NT_109320.5
Ensembl:   ENSMUSG00000063406
Entrez:   73130
Chromosome Mapping:   5|5 E5

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.710000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download