PrimePCR™ SYBR® Green Assay: Olfr779, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCEP0062594

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Olfr779, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Olfr779, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0061445
Assay Design:   exonic
Chromosome Location:   10:129311722-129311892question
Amplicon Length:   141
Splice Variants Targeted:   ENSMUST00000086837 ENSMUST00000071521 ENSMUST00000062353 ENSMUST00000040572 ENSMUST00000069063 ENSMUST00000077024 ENSMUST00000072063 ENSMUST00000077312 ENSMUST00000057775 ENSMUST00000074308 ENSMUST00000082170 ENSMUST00000167055 ENSMUST00000002084 ENSMUST00000177638 ENSMUST00000034281 ENSMUST00000108950 ENSMUST00000042009 ENSMUST00000073687 ENSMUST00000090398 ENSMUST00000040213 ENSMUST00000103108 ENSMUST00000181869

Gene Information

Description Not Available

Gene Symbol:   Olfr779
Gene Name:   Olfactory receptor 779
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSMUSG00000044537
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.998300
y-intercept 35.630000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download