PrimePCR™ SYBR® Green Assay: Hba-a1, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCEP0062646

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Hba-a1, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Hba-a1, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0061497
Assay Design:   exonic
Chromosome Location:   11:32284323-32284424question
Amplicon Length:   72
Splice Variants Targeted:   ENSMUST00000031778 ENSMUST00000055655 ENSMUST00000037380 ENSMUST00000072971 ENSMUST00000135930 ENSMUST00000093209 ENSMUST00000093207 ENSMUST00000119033 ENSMUST00000118952 ENSMUST00000066748 ENSMUST00000172359 ENSMUST00000169686 ENSMUST00000167093 ENSMUST00000033553 ENSMUST00000079322 ENSMUST00000113838 ENSMUST00000048762 ENSMUST00000115349 ENSMUST00000100046 ENSMUST00000039338

Gene Information

Description Not Available

Gene Symbol:   Hba-a1
Gene Name:   Hemoglobin alpha, adult chain 1
Aliases:   Hba, Hba1, Hbat1
RefSeq:   NC_000077.6 NT_039515.7
Ensembl:   ENSMUSG00000069919
Entrez:   15122
Chromosome Mapping:   11 A4|11 16.0 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.998200
y-intercept 36.170000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download