PrimePCR™ SYBR® Green Assay: Psen2, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCIP0031746

List Price:    $179.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Psen2, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Psen2, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0006082
Assay Design:   Intron-spanning
Chromosome Location:   1:180235168-180238912question
Amplicon Length:   120
Splice Variants Targeted:   ENSMUST00000111108 ENSMUST00000111106 ENSMUST00000010753 ENSMUST00000111105 ENSMUST00000111104

Gene Information

This gene encodes a member of the presenilin family. Presenilins are catalytic components of the multi-subunit gamma-secretase complex which mediates critical cellular processes through cleavage of type I transmembrane proteins including Notch receptors and the amyloid precursor protein. The encoded protein contains eight transmembrane domains and is localized to the endoplasmic reticulum where it may play a role in calcium homeostasis. Following assembly of the gamma-secretase complex the encoded protein is cleaved into N- and C-terminal fragments and the activated complex is released from the endoplasmic reticulum. Inactivation of this gene results in impaired synaptic function in a mouse model for Alzheimer's disease. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq Apr 2011]

Gene Symbol:   Psen2
Gene Name:   Presenilin 2
Aliases:   ALG-3, Ad4h, Alg3, PS-2, PS2, Psnl2, STM2
RefSeq:   NC_000067.6 NT_039185.8
Ensembl:   ENSMUSG00000010609
Entrez:   19165
Chromosome Mapping:   1 H4|1 101.0 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.080000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download