PrimePCR™ SYBR® Green Assay: Myc, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCIP0028153

List Price:    $179.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Myc, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Myc, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0006528
Assay Design:   Intron-spanning
Chromosome Location:   15:61988224-61989409question
Amplicon Length:   89
Splice Variants Targeted:   ENSMUST00000161976 ENSMUST00000022971 ENSMUST00000159327 ENSMUST00000167731 ENSMUST00000160009

Gene Information

The protein encoded by this gene is a multifunctional nuclear phosphoprotein that plays a role in cell cycle progression apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations overexpression rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors leukemias and lymphomas including Burkitt lymphoma in human. There is evidence to show that alternative translation initiations from an upstream in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini in human and mouse. Under conditions of stress such as high cell densities and methionine deprivation there is a specific and dramatic increase in the synthesis of the non-AUG initiated protein suggesting its importance in times of adversity. Alternative splicing results in multiple transcript variants. [provided by RefSeq Apr 2010]

Gene Symbol:   Myc
Gene Name:   Myelocytomatosis oncogene
Aliases:   AU016757, Myc2, Niard, Nird, bHLHe39
RefSeq:   NC_000081.6 NT_039621.8
Ensembl:   ENSMUSG00000022346
Entrez:   17869
Chromosome Mapping:   15 D2-D3|15 32.0 cM

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