PrimePCR™ SYBR® Green Assay: Abcg5, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCIP0030694

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Abcg5, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Abcg5, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0012988
Assay Design:   Intron-spanning
Chromosome Location:   17:84669007-84669962question
Amplicon Length:   90
Splice Variants Targeted:   ENSMUST00000066175 ENSMUST00000163375

Gene Information

The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1 MDR/TAP MRP ALD OABP GCN20 White). This protein is a member of the White subfamily. In humans this protein functions as a half-transporter to limit intestinal absorption and promote biliary excretion of sterols; however the function of the mouse gene has not been determined. Mutations in the human gene have been associated with sterol accumulation atheroschlerosis and sitosterolemia. [provided by RefSeq Jul 2008]

Gene Symbol:   Abcg5
Gene Name:   ATP-binding cassette, sub-family G (WHITE), member 5
Aliases:   AW112016, cmp, sterolin-1, trac
RefSeq:   NC_000083.6 NT_039649.8
Ensembl:   ENSMUSG00000040505
Entrez:   27409
Chromosome Mapping:   17 E4|17 54.5 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 34.670000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download