PrimePCR™ SYBR® Green Assay: Oprm1, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qMmuCIP0031542


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PrimePCR™ PreAmp for SYBR® Green Assay: Oprm1, Mouse
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: Oprm1, Mouse
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qMmuCID0014616
Assay Design:		Intron-spanning
Chromosome Location:		10:6789121-6828972question
Amplicon Length:		96
Splice Variants Targeted:		ENSMUST00000105607 ENSMUST00000056385 ENSMUST00000092734 ENSMUST00000105611 ENSMUST00000052751 ENSMUST00000135502 ENSMUST00000105605 ENSMUST00000154906 ENSMUST00000092731 ENSMUST00000000783 ENSMUST00000078634 ENSMUST00000105602 ENSMUST00000105604 ENSMUST00000105603

Gene Information

This gene encodes the mu opioid receptor which is where drugs such as morphine and other opioids have pharmacological effects. Alternatively spliced transcript variants have been found for this gene however many of these variants may be NMD candidates. [provided by RefSeq Jul 2008]

Gene Symbol:		Oprm1
Gene Name:		Opioid receptor, mu 1
Aliases:		M-OR-1, MOP-R, MOR-1, MOR-1O, Oprm, mor, muOR
RefSeq:		NC_000076.6 NT_039490.8
Ensembl:		ENSMUSG00000000766
Entrez:		18390
Chromosome Mapping:		10 A2\|10 8.0 cM

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Mouse Reference Total RNA

Summary Data:

R²	0.998900
y-intercept	35.320000
Efficiency	97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.