PrimePCR™ SYBR® Green Assay: Trim37, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qMmuCIP0030011


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PrimePCR™ PreAmp for SYBR® Green Assay: Trim37, Mouse
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: Trim37, Mouse
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qMmuCID0015005
Assay Design:		Intron-spanning
Chromosome Location:		11:87190097-87196897question
Amplicon Length:		110
Splice Variants Targeted:		ENSMUST00000041282 ENSMUST00000154138

Gene Information

The protein encoded by this gene is part of the tripartite-motif containing family (TRIM) which is typified by the RING B-box type 1 B-box type 2 and coiled-coil region domains. In mouse this protein is proposed to oligomerize through its coiled coil domain and has been reported to be expressed in neural crest-derived tissues as well as in tissues whose development is regulated by mesenchymal-epithelial interactions. In humans mutations in this gene are associated with mulibrey (muscle-liver-brain-eye) nanism an autosomal recessive disorder characterized by prenatal onset growth failure cardiomyopathy and dysmorphic features. [provided by RefSeq Jan 2013]

Gene Symbol:		Trim37
Gene Name:		Tripartite motif-containing 37
Aliases:		1110032A10Rik, 2810004E07Rik, AI848587, AU043018, MUL, TEF3, mKIAA0898
RefSeq:		NC_000077.6 NT_096135.6 NT_187003.1
Ensembl:		ENSMUSG00000018548
Entrez:		68729
Chromosome Mapping:		11 C\|11

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Mouse Reference Total RNA

Summary Data:

R²	0.999900
y-intercept	35.800000
Efficiency	97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.