PrimePCR™ SYBR® Green Assay: Chi3l1, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0015758
Assay Design:   Intron-spanning
Chromosome Location:   1:134183635-134185178question
Amplicon Length:   67
Splice Variants Targeted:   ENSMUST00000156873 ENSMUST00000153856 ENSMUST00000133701 ENSMUST00000132873 ENSMUST00000082060

Gene Information

Description Not Available

Gene Symbol:   Chi3l1
Gene Name:   Chitinase 3-like 1
Aliases:   AW208766, Brp39, Gp39
RefSeq:   NC_000067.6 NT_078297.7
Ensembl:   ENSMUSG00000064246
Entrez:   12654
Chromosome Mapping:   1 E4|1 72.3 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 35.520000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download